The feature of VDJ rearrangment is that to maintain the specificity of recombination, V(D)J recombinase recognizes and binds to recombination signal sequences (RSSs) flanking the variable (V), diversity (D), and joining (J) genes segments. RSSs are composed of three elements: a heptamer of seven conserved nucleotides, a spacer region of 12 or 23 ba
By discovering endogenous non-coding RNA translated peptides and characterizing their functions and structures, scientists are able to make various kinds of exogenous ncRNA which can be translated into exogenous peptides that would be functional in development and cell proliferation and differentiation, or would control or treat some genome malfunc
Agarose Gel Electrophoresis:Materials Required: Buffers and Solutions: Agarose solutions.Ethidium bromide.Electrophoresis buffer. Nucleic Acids and Oligonucleotides: DNA samples.DNA Ladders. (Samples of DNAs of known size are typically generated by restriction enzyme digestion of a plasmid or bacteriophage DNA of known sequence).The equipment and s
Study of miRNA regulating mRNA in your own research baxkground, try to determine if some targeted mRNA interacting with miRNA or not.Wether study of human original or rodent is dependent upon you research backgorund. Try to run luciferase assays, RNA sequencing, and miRNA in situ hybridization.
If the DNA sequences are known, check restriction enzyme sites. Choose those unique enzyme site or sites, make one enzyme digestion of the DNA. Run on the gel, linearal DNA will show one single band, while circled DNA two bands.
Try to make midprep or maxiprep vector plasmid and cut 1 ug of plasmid DNA with BsmB. If BsmB cut linearize the vector, check the digestion on the gel), phenol/chloroform extraction of the digested DNA and ethanol precipitation of the DNA before using it in ligation.Run native PAGE to purify the two oligomers and perform phenol/chloroform extractio